In this paper, we provide a reasonable means for the flow units division and feasibility verification in the sencond area of Dalugou oil reservoir. According to the reservoir flow characteristics, we use the lithology property and physical property method to divide the flow unit, and use discrimination analysis, micro-flow experiment, the production dynamic method, interwell tracer method, and numerical simulation to give a reasonable validation. The results show that: ①The area is divided into four flow units, the distribution of the phases of different flow units is different and they have different physical properties and micro-flow characteristics; ②Integrated with a variety of identification methods of verification, the average correct rate of this division of flow units in this area is 91.4%, and it is feasible; ③B, C-type flow unit, and the combination of several types of flow units is the concentrated area for the remaining oil in the region, and they should be the focus of the potential adjustment; ④The priority direction, which water flow unit combination drives, decides the principle of water injection allocation.