Cathodoluminescence analysis of the carbonate mineral composition and structure is one of the most effective technical methods to research visually and quickly on carbonate reservoir diagenesis environment and its genesis. In this paper，cathodoluminescence features of calcite，filling in Ordovician carbonate fractured-vuggy reservoir，was analyzed further to instruct its diagenetic environment combined with the analysis of Fe-Mn-Sr trace elements and the homogenization temperature of fluid inclusion. Studies have shown that the content of Mn2+ and Fe2+ and cathodoluminescence characteristics largely reflects the diagenetic environment under oxidation-reduction conditions. Bright glow（high Mn2+）suggests the diagenetic environment was under reducing conditions，or it may be related to the stagnant oxide water connected with nonluminance cementation. Dark or non-luminescence suggests the diagenetic environment was under the conditions of oxidation when Mn2+ tended to be oxidized to higher state and difficult to replace Ca2+ and get into the lattice. The luminance of calcite filling in different typical fractured-vuggy reservoir，combined with the content and ratio of Mn2+ and Fe2+，could be used to indicate different diagenetic environment and filling period which show the complexity and multiple phases of the fractured-vuggy forming and filling in the study area.