The favorable synergistic effect of oil displacing agent in alkali-surfactant-polymer（ASP）flooding system can greatly reduce the interfacial tension between oil and water，thus can greatly enhance oil recovery. But the chromatographic separation of ASP flooding system will do great damage to its synergistic effect，which can destroy its integrity. It is important to reveal the chromatographic separation and its influencing factors for improving the oil displacement effect of ASP flooding. Chromatographic separation and its influencing factors of strong-base ASP flooding system were researched in a technical way of chemical analysis and physical simulation guided by reservoir engineering and analytical chemistry theory.The research focuses on geological characteristics and fluid properties of the typical blocks in Daqing. The results show that the sweep volume can be effectively enlarged after using high concentration polymer pre-slug. But the dimensionless dis? tance at the same concentration between surfactant and alkali/polymer is increased，which can make the chromatographic separation phenomenon aggravated. The difference in properties of oil and water of different blocks in Daqing oilfield may have influence on chromatographic separation phenomenon. Because part of heavy alkyl-benzene mahogany acid gets into oil phase，which can influence the concentration measurement value of water phase. In ASP flooding with strong base system，the chromatographic separation degree between surfactant and alkali/polymer is more serious，and that between alkali and polymer is weaker.