Reservoir physical properties and recoveries have a significant impact on the implementation of the endogenous microbial oil displacement technology. However，systematic laboratory research has been lacking in this field，and this technology has been in need of scientific screening criteria of reservoirs. On the basis of Gudong Block 51-511 in Shengli Oilfield，the effects of permeability，surface crude oil viscosity，recovery and heterogeneity on the oil displacement of endogenous microbes were quantitatively studied by physical simulations. When permeability is 50-4 000 mD，the enhanced oil recovery first increases and then decreases. As permeability is 500 mD，the oil displacement effect is the best，and the enhanced oil recovery reaches 6.6%. When the surface crude oil viscosity is within 35-5 371 mPa·s，as the viscosity of crude oil grows，the enhanced oil recovery first rises and then declines. When the surface crude oil viscosity is 1 148 mPa·s，the enhanced oil recovery is the highest，reaching 8.8%. When the recovery is 10%-50%，as it increases，the enhanced oil recovery gradually drops（from 12% to 2%）. When the permeability difference is 2-20，the enhanced oil recovery will decline over time（from 7.3% to 3.2%）as the permeability difference rises. Then the screening criteria of reservoirs with endogenous microbial oil displacement technology are established，and the optimal ranges of key parameters are defined from reservoir，biological，and development indicators. This paper can serve as scientific theoretical reference for reservoir screening by endogenous microbial oil displacement and guide the field implementation.