The characterization of shale oil-bearing properties and shale oil mobility is an important part of shale geological evaluation. The qualitative and quantitative research on pores and fluids on platforms of different scales is mainly carried out by current analytical methods，and cross-scale in-situ accurate evaluation has not yet been realized. Therefore，the mud shale in Dongying Sag of Jiyang Depression was taken as an example，and fluorescence spectroscopy was used to study hydrocarbon inclusions and crude oil in reservoir pores and determine the mathematical relationship between crude oil density and fluorescenc spectrum. Laser confocal technology was also used to accurately identify the properties of point-line-face-volume microscopic crude oil across scales to determine the occurrence state of crude oil in micrometer-nanometer pores. The experimental results show that the fuorescence spectra of aromatic hydrocarbon，non-hydrocarbon，and asphaltene have obvious differences and have good correspondence with crude oil of different densities. The difference in the fluorescence of crude oil is mainly affected by the degree of oil and gas evolution and little affected by the oil and gas differentiation. With the increase in temperature，the alkane and aromatic hydrocarbon increase，and the non-hydrocarbon and asphaltene decrease. The quality of crude oil changes from light to heavy，and the red-green entropy changes from low to high. The fluorescence intensity changes from strong to weak. It can be seen that the red-green entropy and fluorescence intensity of crude oil can be used to characterize crude oil of different densities. In the fourth member of Shahejie Formation（Es₄）of Dongying Sag，The crude oil in calcite veins of mud shale is mainly light oil，and the crude oil in mudstone pores is mostly medium to heavy oil. The crude oil in asphalt and organic matter in mud shale is heavy oil and thickened oil. In addition，the continental mud shale samples in Dongying Sag are highly heterogeneous，and the oil-bearing porosity of the samples varies significantly in the vertical and horizontal sections，indicating that oil and gas are mainly enriched in layers in the interlayers with high porosity. Fluorescence spectroscopy and laser confocal technology can be used to effectively characterize the three-dimensional spatial occurrence characteristics of crude oil in adsorbed and free states in the pores of shale reservoirs. The three-dimensional morphology of crude oil in pores of different lithologies of shale reservoirs was observed in multiple section directions. The crude oil in the clay layer is mainly in an adsorbed state and microscopically appears as intermittent diffuse fog spots，and pore diameter is generally less than 75 nm，with heavy oil dominated. The crude oil in the carbonate layer pore is mainly in a free state and microscopically appears as solid oil columnar. The pore diameters are generally larger than 80 nm，with light-medium oil dominated.