For the interface of different levels, different types, and different acting modes in petroliferous basins, what the petroleum geology study concern most is the different neogenic fluid action interface related to the hydrocarbon fluid generation, expulsion, migration, and accumulation. On the basis of definitions, characteristics, and mode of action analysis of interfaces which includes hydrocarbon generation, hydrocarbon expulsion, the overpressure, secondary pore development, water consumption, this paper presents reservoir-forming acting surface of rift basins. The reservoir-forming acting surface of rift basins is the reflection of coupling of basin neogenic fluid movement process, energy formation and its action mechanism. It is the result of 4 interface coupling that is not only the turnaround surface of fluid process and interfaces of different reservoir spaces, but also the turnaround surface of different energy and reservoir type distribution. Through the control of different fluid potential distribution and superimposition, different reservoir distribution features are affected. By means of identification, research and evaluation of different fluid acting surfaces, the reservoir types and distribution patterns can be predicted effectively. Thus, the reservoir richness of basin can be predicted. It is of great significance for basin evaluation.