Interlayers are one of the main factors controlling the formation and distribution of remaining oil and development performance. Interlayers are controlled and influenced by different geological processes, such as sedimentation and diagenesis. The features,distribution and origin of the interlayers are different, which lead to different rule of oil-water movement. Based on designed ideal model of interlayers, twelve physical experiment models are built. Impact of interlayers on perforations of injection well in rhythmicity reservoir and the impact of distribution, range of the interlayer on water flooding recovery efficiency are studied. It can be concluded that,the injection well should be perforated at high permeability zone for rhythmicity reservoir. And, for reverse rhythm reservoir, when the interlayer is distributed around production well the influence degree on water flooding recovery efficiency is the lowest. As interlayer grows in size in reverse rhythm reservoir, the water flooding recovery efficiency gets worse.