The paper takes one water plugging agent and two different viscosity of profile control agents as research objects. Through the physical simulation experiment on two layers of heterogeneous core, the influence of the sequences and times of profile control and water plugging for enhancing oil recovery were studied. The research results show that: compared with other sequences, profile control before water plugging has better effect, and recovery efficiency has a significant increase. The ultimate recovery of strong gel was 68.5%,and the weak one was 65%. Research on time shows that the longer the interval between profile control and water plugging, the better the effect of oil displacement, and the best interval is 0.5 PV water driving.