Permeability is an important basic parameters in reservoir engineering. First step of conventional method for obtaining permeability is usually to get porosity according to the electric logging interpretation. Second step is to establish petrophysical interpretation model according to the relationship between porosity and permeability obtained from the core laboratory test. And then the permeability is gained. Experimental data of porosity and permeability in 300 pieces of cores of Bohai Y oilfield shows that the permeability in the multi-layer sandstone reservoir cannot be accurately described by unified regression relationship between porosity and permeability，and the permeability from well logging interpretation is often inconsistent with production performance. Therefore，a new method of quantitative characterization based on petrographic constraint，relative permeability test and dynamic agreement was put forward. Microscopically，main factors controlling the relationship between porosity and permeability were known by scanning electron microscope（SEM），cast thin section，logging and core data. Macroscopically，it was verified dynamically by laboratory relative permeability curve，water injection profile and initial productivity using petrographic constraints. The results show that the permeability gained by the new method based on petrographic constraints can improve the alignment with initial injection profile from 65% to 91%.