By means of petrology and geochemical analysis，the oil-bearing properties and material components of shale oil reservoirs in Upper Es4 and Lower Es3 Members in Jiyang Depression were analyzed. Fluorescence characteristics analysis results show that the resident and free asphaltene selectively occur in argillaceous laminates，gray carbonate laminates and fractures. Argillaceous lamina，as the parent material storage for oil-generated，is easy to enrich carbon asphaltene and bituminiferous asphaltene. Carbonate laminates have superior storage space compared with argillaceous laminates，which are suitable to accumulate gum asphaltene and crude oil asphaltene，and crude oil asphaltene can easily flow into microcracks.It is found that the calcite vein is essentially a kind of fully filled fracture. Four identification marks of recognition calcite vein are summarized，among which the calcite intercrystalline pore and the non healing crack near the shaft healing line can be regarded as the percolation path for the oil asphaltene. The spatial development and oil content of shale oil reservoir are mainly controlled by the rock texture，structure types and material components. Mineral composition differences and thermal evolution of organic matter are two main factors that influence shale oil storage space and oil content. As a result，the storage of shale oil has following characteristics：shale with argillaceous laminates and gray carbonate laminates has high porosity，shale with high carbonate content has high porosity，and the higher thermal evolution degree of organic matter corresponds to the higher porosity.